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THIRD PUBLICATION, JANUARY 2016. The Bioscientist. Vol. 4

The Bioscientist: Vol. 4(1): 1-12, January 2016
Available online at http://www.bioscientistjournal.com
Onadeku et al., 2016 Page 1

ACUTE AND CHRONIC EFFECT OF LEAD NITRATE AND CADMIUM SULPHATE IN TADPOLES OF AMIETOPHRYNUS REGULARIS (COMMON AFRICAN TOAD)
*ONADEKO, A. B., AMAEZE, N.H. and OBE, M.A..
Department of Zoology, Faculty of Science, University of Lagos, Akoka, Nigeria

*Corresponding Author’s Email: onadeko2002@yahoo.com or aonadeko@unilag.edu.ng

ABSTRACT (Download PDF)


The relative acute toxicity of cadmium sulphate and lead nitrate acting singly against the
common African toad Amietophrynus regularis were evaluated over a 96 hrs period. The effects
of sub lethal concentrations of the metallic salts on the oxidative responses were also studied
over a 28 day period using whole tadpole homogenate. On the basis of 96 hr LC50 value,
cadmium sulphate with 96 hr LC50 of 1.513mg/l, was found to be 4.3 times more toxic than the
lead salt with 96 hr LC50 of 6.456 mg/l. Exposures to sub lethal concentrations (1/10th and
1/100th 96 hr LC50) of both metals resulted in increase in the levels of lipid peroxidation product,
malondiadehyde (MDA) compared to the control tadpoles at both day 14 and 28. Super oxide
dismutase (SOD) activity was inhibited by day 28 relative to control for both metals but the
activities of catalse (CAT) were often higher than those of the control tadpoles. Lead nitrate
exposed tadpoles had reduced glutathione (GSH) which were significantly inhibited (P<0.05) in
exposed groups in a dose dependent manner compared to the control. The consistency of MDA
levels and SOD activity for both metal salt exposures was in agreement with previous findings
and their combined use as biomarkers of exposure to pollutants in tadpoles is hereby supported.

Keywords: Amietophrynus regularis, metal toxicity, biomarkers, pollutants

© 2013. Journal of Faculty of Biosciences, Nnamdi Azikiwe University,

P. M. B. 5025 Awka, Nigeria.

email: thebioscientist@unizik.edu.ng

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