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FIRST PUBLICATION, JUNE 2013. The Bioscientist. Vol. 1

The Bioscientist: Vol. 1(1):67-72, May, 2013.
Available online at http://www.bioscientistjournal.com
Ezekwesili et al., 2013. Page 67

Evaluation of the pharmacological activity of ethanol leaf extract of Acalypha torta (Muell)
*Ezekwesili, C.N.1 and Nwodo, O.F.C2
1Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
2Department of Biochemistry, University of Nigeria, Nsukka, Nigeria

*Corresponding Author’s Email: cnezekwesili@yahoo.com

ABSTRACT (Download PDF )

The effects of ethanol extract of the leaves of Acalypha torta on spontaneous contraction of isolated
rabbit gut, rat blood platelet aggregation and other haematological indices were investigated. The
height of contraction of the isolated rabbit gut was 0.80 ± 0.03 cm on stabilization. Following the
introduction of A.torta extract (2.5 mg), the height increased to 1.7 ± 0.4(i.e 112.5% increase). Higher
concentrations of the extract ( 5.0, 7.5 and 10.0 mg) did not alter the size of response . Histamine and
acetylcholine standards produced opposite effects on the isolated rabbit gut. While histamine
(0.002μg) abolished contraction, acetylcholine(0.002μg) enhanced contraction. Administration of the
extract (10.0 mg) after incubation with acetylcholine reversed the effect of acetylcholine suggesting
that the extract antagonizes the actions of acetylcholine and may possess antidiarrhoeal potential.
Extract (5.0 mg/ml) also inhibited CaCl2 – induced platelet aggregation by 81.72% (i.e from 87.95 ±
0.16 to 16.08 ± 0.08 % aggregation). Administration of 15.0 mg/ml the extract completely inhibited
the aggregatory effect of CaCl2. This indicates antithrombotic or thrombolytic activity of the extract.
A significant (p < 0.05) increase in haemoglobin concentration (from 12.85 ± 0.1 to 14.43 ± 0.33 g/dl)
was observed at 50.0 mg/kg body wt. of extract. Decreased white blood cell numbers were recorded.

Keywords: Acalypha torta, rabbit smooth muscle, antidiarrheal, antiaggregatory,
thrombolytic, calcium antagonist.

© 2013. Journal of Faculty of Biosciences, Nnamdi Azikiwe University,

P. M. B. 5025 Awka, Nigeria.

email: thebioscientist@unizik.edu.ng

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