PUBLICATION, JANUARY, 2014. The Bioscientist. Vol. 2
The Bioscientist Vol. 2 (1): 41-53, January 2014.
Available online at http://www.bioscientistjournal.com
Ikhajiagbe et al., 2014 Page 41
Heavy metal contents and microbial diversity of waste engine oil-polluted soil in some
public and commercial centres in Benin City metropolis, Nigeria..
Ikhajiagbe B., Chijioke-osuji, C.C and OsazeeJ.O.
1Department of Plant Biology and Biotechnology, University of Benin, Nigeria
2Dept. of Theoretical & Applied Biology, Kwame Nkrumah University of Science & Technology,
*Corresponding author email: firstname.lastname@example.org
ABSTRACT (Download PDF )
This study investigated the heavy metal contents and microbial diversity of waste engine oilpolluted
soil in some public and commercial centres in Benin City metropolis, Nigeria. The study
was carried out in four Local Government Areas (Ikpoba Okha, Ovia North East, Egor and
Oredo) within Benin Metropolis; the administrative headquarters of Edo State of Nigeria. Certain
plots within the study area were selected based on the presence of waste engine oil spill on the
ground, either due to activities of mechanics, parked vehicles, stationary diesel engines, or oil
dump sites. However, in the present study only mechanic workshops (MW), bakeries (BK),
generating plant houses (GN), as well as spare part dealer shops (SP) located within the City
were selected. Field reconnaissance studies were carried to estimate the extent of pollution on
surrounding soil, using the presence and absence of some flora and fauna, and developmental
defects on some flora. Top soil (0 - 10 cm) was randomly collected from about 30 cm from the
periphery of the spill sites and then pooled together to obtain composite samples. These were
labelled appropriately, and immediately transported in polythene bags, covered with aluminium
foil papers, to the laboratory for heavy metal and microbial assay.
Keywords:Hydrocarbons, heavy metal, waste engine oil, bioremediation,
© 2013. Journal of Faculty of Biosciences, Nnamdi Azikiwe University,
P. M. B. 5025 Awka, Nigeria.