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A. A. Jibril
A. Nasiru
Z. Maimuna
M. R. Karima
I. Aminu


This work is aimed at evaluating the inhibitory activities of peptides partially purified from Combretum micranthum (CM)methanol leaf extract against α-glucosidase and α-amylase which are two key enzymes involve in starch degradation and considered as model pathway for new drug discovery for the treatment and prevention of diabetes. Liquid chromatography- Mass Spectroscopy was used to identify the peptides present in the most potent fraction of CM methanol extract and Column chromatography-TLC was used to further fractionate it into sub fractions. The invitro inhibitory activities of the subfractions were carried out using spectrometric method. The results showed a significant (p<0.05) Inhibitory activity of sub fractions A, B and C against α-amylase compared to acarbose as control. Significance (P<0.05) difference was found between acarbose and fractions; A, B and C at all substrate concentration (10, 20, 30, 40, and 50 µg/ml respectively) of CM sub fractions against α-glucosidase. In conclusion, short peptides isolated from C. micranthum have antidiabetic potentials.

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How to Cite
Jibril, A. A., Nasiru, A., Maimuna, Z., Karima, M. R., & Aminu, I. (2022). IN VITRO INHIBITORY ACTIVITIES OF SHORT PEPTIDES PURIFIED FROM COMBRETUM MICRANTHUM AGAINST α-AMYLASE AND α-GLUCOSIDASE. The Bioscientist Journal, 10(3), 326-335. Retrieved from


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