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This work is aimed at evaluating the inhibitory activities of peptides partially purified from Combretum micranthum (CM)methanol leaf extract against α-glucosidase and α-amylase which are two key enzymes involve in starch degradation and considered as model pathway for new drug discovery for the treatment and prevention of diabetes. Liquid chromatography- Mass Spectroscopy was used to identify the peptides present in the most potent fraction of CM methanol extract and Column chromatography-TLC was used to further fractionate it into sub fractions. The invitro inhibitory activities of the subfractions were carried out using spectrometric method. The results showed a significant (p<0.05) Inhibitory activity of sub fractions A, B and C against α-amylase compared to acarbose as control. Significance (P<0.05) difference was found between acarbose and fractions; A, B and C at all substrate concentration (10, 20, 30, 40, and 50 µg/ml respectively) of CM sub fractions against α-glucosidase. In conclusion, short peptides isolated from C. micranthum have antidiabetic potentials.
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