IN VITRO INHIBITORY ACTIVITIES OF SHORT PEPTIDES PURIFIED FROM COMBRETUM MICRANTHUM AGAINST α-AMYLASE AND α-GLUCOSIDASE

Main Article Content

A. A. Jibril
A. Nasiru
Z. Maimuna
M. R. Karima
I. Aminu

Abstract

This work is aimed at evaluating the inhibitory activities of peptides partially purified from Combretum micranthum (CM)methanol leaf extract against α-glucosidase and α-amylase which are two key enzymes involve in starch degradation and considered as model pathway for new drug discovery for the treatment and prevention of diabetes. Liquid chromatography- Mass Spectroscopy was used to identify the peptides present in the most potent fraction of CM methanol extract and Column chromatography-TLC was used to further fractionate it into sub fractions. The invitro inhibitory activities of the subfractions were carried out using spectrometric method. The results showed a significant (p<0.05) Inhibitory activity of sub fractions A, B and C against α-amylase compared to acarbose as control. Significance (P<0.05) difference was found between acarbose and fractions; A, B and C at all substrate concentration (10, 20, 30, 40, and 50 µg/ml respectively) of CM sub fractions against α-glucosidase. In conclusion, short peptides isolated from C. micranthum have antidiabetic potentials.

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How to Cite
Jibril, A. A., Nasiru, A., Maimuna, Z., Karima, M. R., & Aminu, I. (2022). IN VITRO INHIBITORY ACTIVITIES OF SHORT PEPTIDES PURIFIED FROM COMBRETUM MICRANTHUM AGAINST α-AMYLASE AND α-GLUCOSIDASE. The Bioscientist Journal, 10(3), 326-335. Retrieved from https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/128
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