SEROPREVALENCE OF BOVINE BRUCELLOSIS IN CENTRAL CAMEROON
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Abstract
Brucellosis is a zoonotic disease with a significant economic and public health impact, which particularly affects humans and animal species in developing countries relying on livestock production. This study was conducted to provide asero-prevalence of bovine brucellosis in the central part of Cameroon. Sera from randomly collected blood samples were screened for Brucella antibodies using the rapid Rose Bengal Plate Test (RBPT) and the indirect Enzyme Linked Immuno-Sorbent Assay(i-ELISA). A questionnaire was administered to butchers to trace the origin of each animal sampled and know the age and sex of these animals. Statistical significance was determined by Chi-Square test using SPSS v 20 software. A total of 460 cattle (both male and female) were screened. RBPT detected Brucella antibodies in 67 (14.63%) With iELISA, 41 (9, 4%) samples tested positive for detecting Brucella LPS antibodies for confirmation. Data such as animal ageand their origin were not significant; however, the sexwas statistically significant. Animals from Ngaoundere were found to be more affected than animals from Bertoua. Therefore, the overall sero-prevalence obtained was 67 (14.63%) for RBPT and 41 (9, 4%) for i-ELISA.
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Takanouo, D. T., Wade, A., Boda, M., Mbacham, W. F., & Etoa, F. (2023). SEROPREVALENCE OF BOVINE BRUCELLOSIS IN CENTRAL CAMEROON. The Bioscientist Journal, 10(3), 357-366. Retrieved from https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/144
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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Serra,J. and Viñas,M. (2014). Laboratory diagnosis of brucellosis in a rural endemic area in northeastern Spain. International Microbiology, 7(1):53-58.
Shey-Njila, O. (2004). Sero-epidemiological study of bovine brucellosis in the region of Dschang (West, Cameroon).MSc. Thesis ITM Antwerp, Belgium.
Shey-Njila, O.,Awah-Ndukum, J.,Bayemi, P.H.,Nya, E.,Zoli,P.A. and Geerts, S. (2005).Serological survey of bovine brucellosis in Cameroon.Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux 58 (3), 139-143.
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Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M.(1988). “Techniques for the brucellosis laboratory, INRA.” Techniques for the brucellosis laboratory,INRA.
Asgedom,H., Damena,D. and Duguma,R. (2016). Seroprevalence of bovine brucellosis and associated risk factors in and around Alage District, Ethiopia. NCBI PubMed.
Awah-Ndukum, J., Mouiche, M.M.M., Bayang,H.N., Ngu Ngwa, V., Assana, E., Feussom, K.J.M., Manchang, K. and Zoli,P.A. (2018). Seroprevalence and Associated Risk Factors of Brucellosis
among Indigenous Cattle in the Adamawa and North Regions of Cameroon.Veterinary Medicine International,pp. 1-11.
Ayola, M.C., Akinseye,V.O.,Cadmus,E., Awosanya, E., Popoola, O.A., Akinyemi,O.O., Perrett,L., Taylor,A.,Stack, J., Moriyon,I. and Cadmus, S.I. (2017).Prevalence of bovine brucellosis in slaughtered cattle and barriers to better protection of abattoir workers in Ibadan, South-Western Nigeria. Available at:https://www.panafrican-med-journal.com/content/article/28/68.
Baddour,M.M.(2012). Diagnosis of brucellosis in humans: a review. Journal of Veterinary Advances, 2(4):149-156.
Bayemi, P.H., Webb, E.C., Nsongka,M.V., Unger, H. and Njakoi, H. (2008). Prevalence of Brucella abortus antibodies in serum of Holstein cattle in Cameroon.Tropical Animal Health and Production.
Crawford,R.P., Huber,J.D., Adams,B.S., Nielsen,K., and Duncan, J.R. (1990).Epidemiology and Surveillance In: Animal brucellosis. Boca Raton: CRC Press Inc.; pp. 131-148.
Del Pozo,J.S.G., Ortun,S.L., Navarro,E. and Solera,J. (2014). Detection of IgM Antibrucella Antibody in the Absence of IgGs: A Challenge for the Clinical Interpretation of Brucella Serology. PLoS Neglected Tropical Disease,8(12): e3390.
Deshmukh, A., Hagen,F., Sharabasi,O.A., Abraham, M., Wilson, G., Doiphode, S., Maslamani, M.A. and Meis, J.F. (2015).In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar. BMC Microbiology, 15:121.
Fosgate, G.T., Carpenter, T.E., Chomel, B.B., Case, J.T. and DeBess, E.E. (2002). Reilly Time-space clustering of human brucellosis, California, 1973–1992. Emerging Infectious Diseases, 8:672-678.
Ibrahim,N., Belihu,K., Lobago,F. and Bekana,M. (2009). “Seroprevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region, South-western Ethiopia.” TropicalAnimal Health and Production, 42(1):35-40.
Inan,A.S., Ceran, N., Erdem,I., Engin, D.O., Senbayrak, S., Ozyurek,S. and Goktas, P. (2010). Neurobrucellosis with transient ischemic attack vasculopathic changes, intracerebral granulomas and basal ganglia infarction: A case report. The Brazilian Journal of Infectious Diseases.
Limet, J.N., Kerkhofs, P., Wijffels, R. and Dekeyser, P.(1988).“Le diagnostic sérologique de la brucellose bovine par ELISA.”Annales De Medecine Veterinaire, 132:565-575.
Madut, N.A., Muwonge, A., Nasinyama, G.W., Muma, J.B., Godfroid, J., Jubara, A. S., Muleme, J. and Kankya, C. (2018). The sero-prevalence of brucellosis in cattle and their herders in Bahr el Ghazal region, South Sudan. PLoS Neglected Tropical Disease,12(6).
Mai, M.H., Irons,P.C. and Thompson,P.N.A,(2012). Large sero-prevalence survey of brucellosis in cattle herds under diverse production systems in northern Nigeria. BMC Veterinary Research.8 (144).
Mazeri,S., Scolamacchia, F., Handel,I.G., Morgan,K.L., Tanya,V.N. and Bronsvoort,B.M.D.C. (2012). “Risk factor analysis for antibodies to Brucella, Leptospira and Corynebacteria burnetii among cattle in the Adamawa Region of Cameroon: A cross-sectional study.” Tropical Animal Health and Production, 45(1):617-623.
McGiven, J.A. (2013). New developments in the immune-diagnosis of brucellosis in livestock and wildlife. Review Science Technology, 32(1):163-176.
Mohamed,E.M., Heba,A.A., Ashraf, E.S., Fatma,I.E., Tahoun Asmaa,B.M.B. and Saleh,M.S. (2016). Seroprevalence of Brucella spp. in Cattle, Molecular Characterisation in Milk, and the Analysis of Associated Risk Factors with Seroprevalence in Humans, Egypt.Vector-Borne and Zoonotic Diseases,16(12):758-764.
Mufinda, F.C., Boinas,F. and Nunes, C. (2017).Prevalence and factors associated with human brucellosis in livestock professionals. Review Saude Publica.
McDermott,J.J. and Arimi,S.M. (2002). Brucellosis in Sub-Saharan Africa: epidemiology, control, and impact. Veterinary Microbiology.
Muma,J.B., Samui,K.L., Oloya, J., Munyeme,M. and Skjerve, E. (2007). “Risk factors for brucellosis in indigenous cattle reared in livestock-wildlife interface areas of Zambia.” PreventiveVeterinary Medicine, 80(4):306-317.
OIE (2009). Bovine brucellosis: Terrestrial Manual, Chapter 2.4.3, Version adopted by the World Assembly of Delegates of the OIE; pp. 1-35.
Ojong,B.,Macleod,E., Ndip,M.L., Zuliani, A. and Piasentier,E. (2016). Brucellosis in Cameroon: Seroprevalence and risk factors in beef-type cattle. International Journal of Infectious Diseases, 53:64.
Percy,A.,D. Gautier, O. Lecointre, D. Crevat. (1998). Diagnostic sérologique de la brucellose bovine : Evaluation comparative d’une méthode ELISA et des méthodes traditionnelles. Revue d’élevage et de médecine vétérinaire des pays tropicaux. 149:161-168.
Salman, A.M.A., Mustafa, E.A., Hamid, A.M. and Hassan, L.M. (2014). Application of Different Serological Tests for the Detection of the Prevalence of Bovine Brucellosis in Lactating Cows in Khartoum State, Sudan. Journal of Applied and Industrial Sciences, 2(5):213-218.
Scolamacchia, F., Handel, I.G., F`evre, E.M., Morgan, K.L., Tanya, V.N. and Bronsvoort, B.M.D.C. (2010). “Serological patterns of brucellosis, leptospirosis and Q fever in Bos indicus cattle in Cameroon.” PLoS ONE, 5(1). Article ID e8623. 2010.
Serra,J. and Viñas,M. (2014). Laboratory diagnosis of brucellosis in a rural endemic area in northeastern Spain. International Microbiology, 7(1):53-58.
Shey-Njila, O. (2004). Sero-epidemiological study of bovine brucellosis in the region of Dschang (West, Cameroon).MSc. Thesis ITM Antwerp, Belgium.
Shey-Njila, O.,Awah-Ndukum, J.,Bayemi, P.H.,Nya, E.,Zoli,P.A. and Geerts, S. (2005).Serological survey of bovine brucellosis in Cameroon.Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux 58 (3), 139-143.
Singh, D., Somani, V.K.,Aggarwal, S. and Bhatngar, R. (2015). RPLGA (85:15)nanoparticle-based delivery of Rl7/L12 ribosomal protein in mice protects against Brucella abortus 544 infections: a promising alternate to traditional adjuvants.Molecular Immunology, 68:272-279.
Tasiame,W., Emikpe,B.O., Folitse,R.D., Fofie, C.O., Burimuah,V., Johnson,S.,Awuni,J.A., Afari,E., Yebuah, N. and Wurapa,F. (2016). The prevalence of brucellosis in cattle and their handlers in north tongu district of Volta Region, Ghana. Africa Journal of Infectious Diseases,10(2):111-117.
WHO (2007). World Health Organization Integrated control of Neglected Zoonotic Diseases in Africa.ILRI Headquarters. Nairobi Kenya.