AMELIORATING ACTION OF CNIDOSCOLUS ACONITIFOLIUS ON TESTOSTERONE PROPIONATE-INDUCED BENIGN PROSTATE HYPERPLASIA RATS
Main Article Content
Abstract
Unhealthy lifestyle, high cost of living, non-balance feeding habit and poor exercise are among the major contributory factors to high incidence of benign prostate hyperplasia especially among men of 40 years and above. Improvement of biochemical parameters in testosterone propionate-induced benign prostate hyperplasia (BPH) rats by Cnidoscolus aconitifolius was studied. The 28days study was done using 30 male albino rats grouped into six with 5 rats each. The extraction and characterization of the leaves were done using standard methods. Enzyme assays and other biochemical parameters were determined using spectrophotometric techniques. The results obtained revealed that the administration of Cnidoscolus aconitifolius significantly reduced the Prostate Specific Antigen (PSA), Dihydrotestosterone (DHT), Alkaline phosphatase (ALP) and Inflammatory marker levels when compared with the BPH (negative) control group whose values were elevated. The histopathology assays of the treated groups showed little healing in the prostate as compared to the negative control group. The results suggest that Cnidoscolus aconitifolius possesses anti-BPH potentials and may be encouraged for management of BPH.
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Nwobodo, V. O., Ibiam, U. A., Obiekezie, T. O., Chigbo, C. M., Osita, B. N., & Nwarienne, C. M. (2024). AMELIORATING ACTION OF CNIDOSCOLUS ACONITIFOLIUS ON TESTOSTERONE PROPIONATE-INDUCED BENIGN PROSTATE HYPERPLASIA RATS. The Bioscientist Journal, 12(1), 59-75. https://doi.org/10.54117/the_bioscientist.v12i1.166
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This work is licensed under a Creative Commons Attribution 4.0 International License.
References
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Al-Trad, B., Aljabali, A., Al Zoubi, M., Shehab, M. and Omari, S. (2019). Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats. International Journal of Nanomedicine, 14: 31-45.
Batista, R. L. and Mendonca, B. B. (2022). The Molecular Basis of 5α-Reductase Type 2 Deficiency. Sex Development, 16:171-183.
Bhattacharjee, P. and Bhattacharyya, D. (2013). Characterization of the aqueous extract of the root of Aristolochia indica: Evaluation of its traditional use as an antidote for snake bites. Journal of Ethnopharmacology, 145: 220–226.
Cai, H., Zhang, G., Yan, S. and Shang, X. (2018). The Effect of Xialiqi Capsule on Testosterone-Induced Benign Prostatic Hyperplasia in Rats. Evidence-Based Complementary and Alternative Medicine, 18: 1-9.
Chen, Z., Prestigiacomo A. F. and Stamey, T. A. (1995). Purification and characterization of prostate specific antigen (PSA) complexed to chymotrypsin: potential reference material for International Standardization of PSA Immunoassays. Clinical Chemistry, 41(9): 1273-1282.
Csikos, E., Horvath, A., Acs, K., Papp, N., Balazs, V. L., Dolenc, M. S., Kenda, M., Glavac, N. K., Naggy, M., Protti, M., Mercolini, L., Horvath, G. and Farkas, A. (2021). Treatment of Benign Prostatic Hyperplasia by Natural Drugs. Molecules, 26: 7141.
De Nunzio, C., Presicce, F. and Tubaro, A. (2016). Inflammatory mediators in the development and progression of benign prostatic hyperplasia. Nature Review on Urology, 13:613–626
Delvin, C. M., Simms, M. S. and Maitland, J. N. (2019). Benign prostatic hyperplasia- what do we know? British Journal of Urology, 127: 389-399.
Deters, A. L. (2021). Benign Prostatic Hyperplasia (BPH) Treatment & Management. Medscape, 23: 15-17.
Edmond, G.E., Gnalen, D.M, Kévin, K., Cyrille, V.C., Gérard, K.P. and Hygin, D.A. (2023). Complications of Surgery for Benign Prostate Hyperplasia (BPH) in the Urology Department of University Hospital of Cocody. International Archives of Urology and Complications, 9:1-5.
Eleazu, K., Aja, P.M. and Eleazu, C.O. (2021): Cocoyam (Colocasia esculenta) modulates some parameters of testosterone propionate-induced rat model of benign prostatic hyperplasia. Drug and Chemical Toxicology, 10:1-12.
Ezeigwe, O.C., Okpala, C.O., Ogana, J., Akonam, N.E., Aziagba, B.O., Nnadi, N.N., Anekwe, O.J. and Nwobodo, V.O.G. (2020). Comparative phytochemical and Nutritional profiles of Ficuscapensis and Cnidoscolus aconitifolius leaves. International Journal of Research and Innovation in Applied Science, 5(1): 16-21.
Hoffman, M. (2020). Picture of the Prostate - Human Anatomy. The Medical Web, 3: 2-5.
Ijioma, S.N., Nwosu, C.O., Emelike, C.U., Okafor, A.I. and Nwankwo, A.A. (2014). Antinociceptive property of Costus afer Ker stem juice and ethanol leaf extract in albino rats. Comprehensive Journal of Medical Sciences, 2(2): 14 -19.
Ishola, I.O., Yemitan, K.O., Afolayan, O.O., Anunobi, C.C. and Durojaiye, T.E. (2017). Potential of Moringaoleifera in the Treatment of Benign Prostate Hyperplasia: Role of Antioxidant Defence Systems. Journal of Medical Principles and Practice, 27:15–22.
Jang, D., Lee, A., Shin, H., Song, H., Park, J., Kang, T., Lee, S. and Yang, S. (2021). The Role of Tumor Necrosis Factor Alpha in Autoimmune Diseaases and Current TNF- α Inhibitors in Therapeutics. International Journal of Molecular Sciences, 22(5): 2719.
Jeon, Y.J., Kim, B.H., Kim, S., Oh, I., Lee, S., Shin, J. and Kim, T.Y. (2013). Rhododendrin ameliorates skin inflammation through inhibition of NF-κB, MAPK, and PI3K/Akt signaling. European Journal of Pharmacology, 714: 7–14.
Kim, C., Chung, K., Cheon, S., Lee, J., Park, Y. and An, H. (2016). Rice Hull Extract Suppresses Benign Prostate Hyperplasia by Decreasing Infammation and Regulating Cell Proliferation in Rats. Journal of Medicinal Food, 19(8): 746–754.
Klin, Z. (1980). Chemistry. Klin Biochemistry, 8:658.
Lokeshwar, D. S., Harper, T. B., Webb, E., Jordan, A., Dykes, T. A., Neal, E. D., Terris, K. M. and Klaassen, Z. (2019). Epidemiology and treatment modalities for the management of benign prostatic hyperplasia. Translational Andrology and Urology, 8(5): 529-539.
Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54: 275–287.
Madersbacher, S., Sampson, N. and Culig, Z. (2019). Pathophysiology of Beingn Prostatic Hyperplasia and Benign Prostatic Enlargement: A Mini-Review. Gerontology, 65:458-464.
McLaren, L.D., Jerde, T.J. and Bushman, W. (2011). Role of interleukins, IGF and stem cells in BPH. Differentiation, 82: 237–243.
Mominur, R., Saidur, R., Rezaul, I., Firoza, R., Faria, M. M., Taha, A., Mohannad, A. A., Samia, Q. A. and Abdullah, S. A. (2021). Role of Phenolic Compounds in Human Disease: Current Knowledge and Future Prospects. Multidisciplinary Digital Publishing Institute, 27(1): 233.
Obisike, U.A., Nwachukwu, E.O., Boisa, N. and Nduka, N. (2019). Determination of Exogenous Testosterone Propionate Dose for Induction of Benign Prostatic Hyperplasia in Rat Model. European Journal of Biomedical and Pharmaceutical Sciences, 6(13): 141-147.
Oluoch, C.O., Ikol, J., Mburugu, G., Gachie, M. and Inyangala, D. (2023). Benign Prostatic Hyperplasia in a 13-Year-Old Boy. Annals of African Surgery, 20(2): 51-57.
Panghal, A., Shaji, A.O., Nain, K., Garg, M.K. and Chhikara, N. (2021). Cnidoscolus aconitifolius: Nutritional, phytochemical composition and health benefits- A review. Bioactive Compounds in Health and Disease, 4(11): 260-286.
Prakash, A. R., Nahar, P. and Ashtekar, M. (2020). Serum and salivary alkaline phosphatase levels in oral health and disease: A brief review. IP International Journal of Maxillofacial Imaging, 6(3): 53-55.
Praveen, R. (2013). Benign prostate hyperplasia: Updated review. International Research Journal of Pharmacy, 4(8): 45-51.
Ross, P. (2021). Medications for Benign Prostatic Hyperplasia. Neuroscience and Therapeutics, 10: 23.
Schenk, J.M., Kristal, A.R., Neuhouser, M.L., Tangen, C.M., White, E., Lin, D.W., Kratz, M. and Thompson, I.M. (2010). Biomarkers of systemic inflammation and risk of incident, symptomatic benign prostatic hyperplasia: results from the prostate cancer prevention trial. American Journal of Epidemiology, 171: 571–582.
Shabani, E., Kalantari, H., Kalantar, M., Goudarzi, M., Mansouri, E. and Kalantar, H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BioMed Central Complementary Medicine and Therapies, 21: 301.
Somade, O. T., Ugbaja, R. N., Idowu, M. A. and Akinloyea, A. O. (2021). Cnidoscolus aconitifolius leaf extract and ascorbate confer amelioration and protection against dimethyl nitrosamine-induced renal toxicity and testicular abnormalities in rats. Toxicology Reports, 8:1098–1108.
Stamey T.A., McNeal, J.E., Yemoto, C.M., Sigal, B.M. and Johnstone, I.M. (1999). Biological determinants of cancer progression in men with prostate cancer. Journal of American Medical Association, 281:1395-1400.
Steiner, G.E., Newman, M.E., Paikl, D., Stix, U., Memaran-Dagda, N., Lee, C. and Marberger, M.J. (2002). Expression and function of pro-inflammatory interleukin IL-17 and IL-17 receptor in normal, benign hyperplastic, and malignant prostate. Prostate, 56: 171–182.
Traish, A. M., Hassani, J., Guay, A. T., Zitzmann, M. and M. Hansen, L. (2011). Adverse Side Efects of 5α-Reductase Inhibitors Therapy: Persistent Diminished Libido and Erectile Dysfunction and Depression in a Subset of Patients. Journal of Sexual Medicine, 8(3):872-884.
Tsai, S. (2021). Role of interleukin8 in depression and other psychiatric disorders. Progress inNeuro-Psychopharmacology and Biological Psychiatry, 106: 110173.
Tusubira, D., Aja, P.M., Munezero, J., Ssedyabane, F., Namale, N., Ifie, J.E., Agu, P.C., Ajayi, C.O. and Okoboi, J. (2023). Safety profile of colocasia esculenta tuber extracts in benign prostate hyperplasia. BMC Complementary Medicine and Therapies, 23: 187-200.
Vafa, A., Afzal, S. M., Barnwal, P., Rashid, S., Shahid, A., Alpashree, J. I. and Sultana, S. (2020). Protective role of diosmin against testosterone propionate-induced prostatic hyperplasia in Wistar rats: plausible role of oxidative stress and inflammation. Human & Experimental Toxicology, 39(9): 1133-1146.
Veeresh-Babu, S.V., Veeresh, B., Patil, A.A. and Warke, Y.B. (2010). Lauric acid and myristic acid prevent testosterone induced prostatic hyperplasia in rats. European Journal of Pharmacology, 626: 262–265.
Wang, M., Liu, X., Chen, Z., Chen, H. and Tan, Y. (2020). Metformin suppressed tumor necrosis factor-α-induced epithelial-mesenchymal transition in prostate cancer by inactivating the NF-B signaling pathway. Translational Cancer Research, 9(10): 6086-6095.
Yang, B., Jin, L., Yang, Y., Li, K. and Peng, D. (2014a). Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone induced benign prostatic hyperplasia in rats. Experimental and Therapeutic Medicine, 8: 31-37.
Yang, X, Yuan, L., Xiong, C., Yin, C. and Ruan, J. (2014b). Abacopterispenangiana exerts testosterone-induced benign prostatic hyperplasia protective effect through regulating inflammatory responses, reducing oxidative stress and anti-proliferative. Journal of Ethnopharmacology, 157: 105–113.
Zaher, D. M., El-Gamel, M. I., Omar, H. A., Aljareh, S. N., Al-Shamma, S. A., Aya, J. A., Zaib, S. and Iqbal, J. (2020). Recent advances with alkaline phosphatase isoenzymes and their inhibitors. Archives of Pharmacal Research, 353(5): e2000011.
Al-Trad, B., Aljabali, A., Al Zoubi, M., Shehab, M. and Omari, S. (2019). Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats. International Journal of Nanomedicine, 14: 31-45.
Batista, R. L. and Mendonca, B. B. (2022). The Molecular Basis of 5α-Reductase Type 2 Deficiency. Sex Development, 16:171-183.
Bhattacharjee, P. and Bhattacharyya, D. (2013). Characterization of the aqueous extract of the root of Aristolochia indica: Evaluation of its traditional use as an antidote for snake bites. Journal of Ethnopharmacology, 145: 220–226.
Cai, H., Zhang, G., Yan, S. and Shang, X. (2018). The Effect of Xialiqi Capsule on Testosterone-Induced Benign Prostatic Hyperplasia in Rats. Evidence-Based Complementary and Alternative Medicine, 18: 1-9.
Chen, Z., Prestigiacomo A. F. and Stamey, T. A. (1995). Purification and characterization of prostate specific antigen (PSA) complexed to chymotrypsin: potential reference material for International Standardization of PSA Immunoassays. Clinical Chemistry, 41(9): 1273-1282.
Csikos, E., Horvath, A., Acs, K., Papp, N., Balazs, V. L., Dolenc, M. S., Kenda, M., Glavac, N. K., Naggy, M., Protti, M., Mercolini, L., Horvath, G. and Farkas, A. (2021). Treatment of Benign Prostatic Hyperplasia by Natural Drugs. Molecules, 26: 7141.
De Nunzio, C., Presicce, F. and Tubaro, A. (2016). Inflammatory mediators in the development and progression of benign prostatic hyperplasia. Nature Review on Urology, 13:613–626
Delvin, C. M., Simms, M. S. and Maitland, J. N. (2019). Benign prostatic hyperplasia- what do we know? British Journal of Urology, 127: 389-399.
Deters, A. L. (2021). Benign Prostatic Hyperplasia (BPH) Treatment & Management. Medscape, 23: 15-17.
Edmond, G.E., Gnalen, D.M, Kévin, K., Cyrille, V.C., Gérard, K.P. and Hygin, D.A. (2023). Complications of Surgery for Benign Prostate Hyperplasia (BPH) in the Urology Department of University Hospital of Cocody. International Archives of Urology and Complications, 9:1-5.
Eleazu, K., Aja, P.M. and Eleazu, C.O. (2021): Cocoyam (Colocasia esculenta) modulates some parameters of testosterone propionate-induced rat model of benign prostatic hyperplasia. Drug and Chemical Toxicology, 10:1-12.
Ezeigwe, O.C., Okpala, C.O., Ogana, J., Akonam, N.E., Aziagba, B.O., Nnadi, N.N., Anekwe, O.J. and Nwobodo, V.O.G. (2020). Comparative phytochemical and Nutritional profiles of Ficuscapensis and Cnidoscolus aconitifolius leaves. International Journal of Research and Innovation in Applied Science, 5(1): 16-21.
Hoffman, M. (2020). Picture of the Prostate - Human Anatomy. The Medical Web, 3: 2-5.
Ijioma, S.N., Nwosu, C.O., Emelike, C.U., Okafor, A.I. and Nwankwo, A.A. (2014). Antinociceptive property of Costus afer Ker stem juice and ethanol leaf extract in albino rats. Comprehensive Journal of Medical Sciences, 2(2): 14 -19.
Ishola, I.O., Yemitan, K.O., Afolayan, O.O., Anunobi, C.C. and Durojaiye, T.E. (2017). Potential of Moringaoleifera in the Treatment of Benign Prostate Hyperplasia: Role of Antioxidant Defence Systems. Journal of Medical Principles and Practice, 27:15–22.
Jang, D., Lee, A., Shin, H., Song, H., Park, J., Kang, T., Lee, S. and Yang, S. (2021). The Role of Tumor Necrosis Factor Alpha in Autoimmune Diseaases and Current TNF- α Inhibitors in Therapeutics. International Journal of Molecular Sciences, 22(5): 2719.
Jeon, Y.J., Kim, B.H., Kim, S., Oh, I., Lee, S., Shin, J. and Kim, T.Y. (2013). Rhododendrin ameliorates skin inflammation through inhibition of NF-κB, MAPK, and PI3K/Akt signaling. European Journal of Pharmacology, 714: 7–14.
Kim, C., Chung, K., Cheon, S., Lee, J., Park, Y. and An, H. (2016). Rice Hull Extract Suppresses Benign Prostate Hyperplasia by Decreasing Infammation and Regulating Cell Proliferation in Rats. Journal of Medicinal Food, 19(8): 746–754.
Klin, Z. (1980). Chemistry. Klin Biochemistry, 8:658.
Lokeshwar, D. S., Harper, T. B., Webb, E., Jordan, A., Dykes, T. A., Neal, E. D., Terris, K. M. and Klaassen, Z. (2019). Epidemiology and treatment modalities for the management of benign prostatic hyperplasia. Translational Andrology and Urology, 8(5): 529-539.
Lorke, D. (1983). A new approach to practical acute toxicity testing. Archives of Toxicology, 54: 275–287.
Madersbacher, S., Sampson, N. and Culig, Z. (2019). Pathophysiology of Beingn Prostatic Hyperplasia and Benign Prostatic Enlargement: A Mini-Review. Gerontology, 65:458-464.
McLaren, L.D., Jerde, T.J. and Bushman, W. (2011). Role of interleukins, IGF and stem cells in BPH. Differentiation, 82: 237–243.
Mominur, R., Saidur, R., Rezaul, I., Firoza, R., Faria, M. M., Taha, A., Mohannad, A. A., Samia, Q. A. and Abdullah, S. A. (2021). Role of Phenolic Compounds in Human Disease: Current Knowledge and Future Prospects. Multidisciplinary Digital Publishing Institute, 27(1): 233.
Obisike, U.A., Nwachukwu, E.O., Boisa, N. and Nduka, N. (2019). Determination of Exogenous Testosterone Propionate Dose for Induction of Benign Prostatic Hyperplasia in Rat Model. European Journal of Biomedical and Pharmaceutical Sciences, 6(13): 141-147.
Oluoch, C.O., Ikol, J., Mburugu, G., Gachie, M. and Inyangala, D. (2023). Benign Prostatic Hyperplasia in a 13-Year-Old Boy. Annals of African Surgery, 20(2): 51-57.
Panghal, A., Shaji, A.O., Nain, K., Garg, M.K. and Chhikara, N. (2021). Cnidoscolus aconitifolius: Nutritional, phytochemical composition and health benefits- A review. Bioactive Compounds in Health and Disease, 4(11): 260-286.
Prakash, A. R., Nahar, P. and Ashtekar, M. (2020). Serum and salivary alkaline phosphatase levels in oral health and disease: A brief review. IP International Journal of Maxillofacial Imaging, 6(3): 53-55.
Praveen, R. (2013). Benign prostate hyperplasia: Updated review. International Research Journal of Pharmacy, 4(8): 45-51.
Ross, P. (2021). Medications for Benign Prostatic Hyperplasia. Neuroscience and Therapeutics, 10: 23.
Schenk, J.M., Kristal, A.R., Neuhouser, M.L., Tangen, C.M., White, E., Lin, D.W., Kratz, M. and Thompson, I.M. (2010). Biomarkers of systemic inflammation and risk of incident, symptomatic benign prostatic hyperplasia: results from the prostate cancer prevention trial. American Journal of Epidemiology, 171: 571–582.
Shabani, E., Kalantari, H., Kalantar, M., Goudarzi, M., Mansouri, E. and Kalantar, H. (2021). Berberine ameliorates testosterone-induced benign prostate hyperplasia in rats. BioMed Central Complementary Medicine and Therapies, 21: 301.
Somade, O. T., Ugbaja, R. N., Idowu, M. A. and Akinloyea, A. O. (2021). Cnidoscolus aconitifolius leaf extract and ascorbate confer amelioration and protection against dimethyl nitrosamine-induced renal toxicity and testicular abnormalities in rats. Toxicology Reports, 8:1098–1108.
Stamey T.A., McNeal, J.E., Yemoto, C.M., Sigal, B.M. and Johnstone, I.M. (1999). Biological determinants of cancer progression in men with prostate cancer. Journal of American Medical Association, 281:1395-1400.
Steiner, G.E., Newman, M.E., Paikl, D., Stix, U., Memaran-Dagda, N., Lee, C. and Marberger, M.J. (2002). Expression and function of pro-inflammatory interleukin IL-17 and IL-17 receptor in normal, benign hyperplastic, and malignant prostate. Prostate, 56: 171–182.
Traish, A. M., Hassani, J., Guay, A. T., Zitzmann, M. and M. Hansen, L. (2011). Adverse Side Efects of 5α-Reductase Inhibitors Therapy: Persistent Diminished Libido and Erectile Dysfunction and Depression in a Subset of Patients. Journal of Sexual Medicine, 8(3):872-884.
Tsai, S. (2021). Role of interleukin8 in depression and other psychiatric disorders. Progress inNeuro-Psychopharmacology and Biological Psychiatry, 106: 110173.
Tusubira, D., Aja, P.M., Munezero, J., Ssedyabane, F., Namale, N., Ifie, J.E., Agu, P.C., Ajayi, C.O. and Okoboi, J. (2023). Safety profile of colocasia esculenta tuber extracts in benign prostate hyperplasia. BMC Complementary Medicine and Therapies, 23: 187-200.
Vafa, A., Afzal, S. M., Barnwal, P., Rashid, S., Shahid, A., Alpashree, J. I. and Sultana, S. (2020). Protective role of diosmin against testosterone propionate-induced prostatic hyperplasia in Wistar rats: plausible role of oxidative stress and inflammation. Human & Experimental Toxicology, 39(9): 1133-1146.
Veeresh-Babu, S.V., Veeresh, B., Patil, A.A. and Warke, Y.B. (2010). Lauric acid and myristic acid prevent testosterone induced prostatic hyperplasia in rats. European Journal of Pharmacology, 626: 262–265.
Wang, M., Liu, X., Chen, Z., Chen, H. and Tan, Y. (2020). Metformin suppressed tumor necrosis factor-α-induced epithelial-mesenchymal transition in prostate cancer by inactivating the NF-B signaling pathway. Translational Cancer Research, 9(10): 6086-6095.
Yang, B., Jin, L., Yang, Y., Li, K. and Peng, D. (2014a). Inhibitory effect of rape pollen supercritical CO2 fluid extract against testosterone induced benign prostatic hyperplasia in rats. Experimental and Therapeutic Medicine, 8: 31-37.
Yang, X, Yuan, L., Xiong, C., Yin, C. and Ruan, J. (2014b). Abacopterispenangiana exerts testosterone-induced benign prostatic hyperplasia protective effect through regulating inflammatory responses, reducing oxidative stress and anti-proliferative. Journal of Ethnopharmacology, 157: 105–113.
Zaher, D. M., El-Gamel, M. I., Omar, H. A., Aljareh, S. N., Al-Shamma, S. A., Aya, J. A., Zaib, S. and Iqbal, J. (2020). Recent advances with alkaline phosphatase isoenzymes and their inhibitors. Archives of Pharmacal Research, 353(5): e2000011.