Determination of β–Carotene & Vitamin C content of Fresh Green Pepper (Capsicum annnum), Fresh Red Pepper (Capsicum annum) and Fresh Tomatoes (Solanumly copersicum) Fruits

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G.E. Igbokwe
C.O. Anagonye


The interest in the consumption of different varieties of pepper fruits, (Capsicum annuum) and also tomato fruit (Solanum lycopersicum) is to a large extent, due to their content of bioactive nutrients and their importance as dietary antioxidants. A laboratory experiment was carried out to determine the β– carotene and vitamin C content of green pepper fruit, red pepper fruit and tomato fruit. The β–carotene content of the methanolic extract of the three samples was determined spectrophotometrically. Vitamin C content of the fruits was measured as ascorbic acid by titrating the aqueous mixtures of the samples. The result showed that tomato has the highest β–carotene content (896μg/100ml of extract) and the lowest vitamin C content (54.12mg/100g) while red pepper has the highest vitamin C content (126.05mg/100g) and considerable high amount of β–carotene (592μg/100ml of extract). Green pepper on the other hand, has a considerable high amount of vitamin C (116.08mg/100g) and β–carotene content of 368μg/100ml of extract). From the nutritional point of view, the data in this report will serve as a useful guide to dieticians in the selection of plants which are rich in these antioxidants.

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Igbokwe, G., & Anagonye, C. (2013). Determination of β–Carotene & Vitamin C content of Fresh Green Pepper (Capsicum annnum), Fresh Red Pepper (Capsicum annum) and Fresh Tomatoes (Solanumly copersicum) Fruits . The Bioscientist Journal, 1(1), 89-93. Retrieved from


Aniakor, (2011).Determination of β–carotene & vitamin C content of Fresh Green pepper (Capsicum annuum), Fresh red pepper (Capsicum annuum) & Fresh Tomatoes (Solanum lycoperisicum) fruits. Unpublished B.Sc thesis submitted to Dept of Applied Biochemistry, Nnamdi Azikiwe
University, Awka, Nigeria.

Barros, L; Ferreira, M. J, Queiros, B, Ferreira, I. C. F. R and Baptista P (2007). Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food chemistry, 103(2): 413419.

Cai-Xia Zhang; Suzanne C. Ho, Yu-ming Chen, Zian-Huafu; Shou-Zhen Cheng and Frang-Yulin (2009). Greater vegetable and fruit intake is associated with a lower risk of breast cancer among
Chinese women. International Journal of cancer 125(1): 181-8.

Collins, A. R (2005). “Assay for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenols”. American J. of Clinical Nutri. 81:2615-2675.

Dodukan A, Tuzui M, Agca CA et al (2011). A tomato lycopene comple proteins the kidney from cisplatin-induced injury via affecting oxidative stress as well as Bax, Bcl-2, and HSPs expression. Nutr cancer 63(3): 427-34.

Grubben, G. J. H and Denton, O. A (2004). Plant Resources of Tropical Africa 2 vegetables. PROTAF Foundation, Wageningen, Backhuys, Leiden, CTA, Wageningen.

Harris, J. R (1996). “Sub cellular biochemistry, ascorbic acid”. Biochemistry and biomedical cell biology (vol. 25). New York. Plenum.

Howard, L. R.., Talcott, S. T., Brenes, C.H., and
Villalon, B (2000). “Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum annuum) and as influenced by maturity. J. of Agric Food Chemistry.48: 17131720.

HU, F.B (2003). “Plant-based food and prevention of cardiovascular disease: an overview”. American J. of Clinical Nutri. 78:5445-5515.

Josefa, M., Navarro, Pilar, F., Consuelo, G., Vicente, M (2005). “Changes in the content of antioxidant compounds in pepper fruits at different ripening stages, as affected by salinity”.J. of Agric. And Food Chemistry. 96(10): 66-73.

Lathan, Elizabeth (2009). The Colourful World of Chilies. Retrieved 2009.

Nagata, M. and Yamashita, I (1992). “Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit”. J. of Japanese Society of Food Sci. and Technil.39:925-928.

Namiki, M (1990). “Antioxidants/antimutagens in food.CRC critical Reviews in Food Sci. and Nutri.29:273-300.

Ogunlesi, M., Okiei, W., Ademoye, M (2008).
“A Textbook of Medicinal Plants from Nigeria”, University of Lagos Press, Lagos. In: T. Odugbemi (ed). Pp 299.

Ogunlesi, M., Okiei, W., Azeez, L., Obakachi, V., Osunsanmi, M., Nkenchor, G.
(2010). “Vitamin C contents of tropical vegetable and fruits determined by voltameric and titrimeric methods and their relevance to the medicinal uses of plants”. Int. J. Electrochem. Sci. 5:105115.

Shidfar, F; Froghifar, N; Vafa, M; Rajab, A; Hosseini, S; Shidfar, S; and Gohari, M (2011). The effects of tomato consumption on serum glucose, apolipoprotein B, apolipoprotein A-1, homocysteine and blood pressure in type 2 diabetic patients. Int. J. Food Sci Nutr.

Venket Rao A; and Sanjio Agarwal (2000). Role of Antioxidant lycopene in Cancer and Heart Disease. J. Am.coll. Nutr 19(5): 563-569