SURVEY OF GASTROINTESTINAL AND PULMONARY PARASITISM IN TWO LOCAL CATTLE BREEDS IN THE HIGH GUINEA SAVANNAH, CAMEROON

Main Article Content

N. J. Ebene
D. Njonka
I. O. Onyali
T. K. Manchang
O. O. Ikpeze

Abstract

Cattle production accounts for over sixty percent of meat requirement in Cameroon but helminthosis greatly hinders its productivity. A study on worm burdens in digestive and pulmonary organs of cattle was done between February 2018 and January 2019 at a Research Centre in Cameroon with fourteen apparently healthy adult cattle that were  necropsied. Recovered worms were adequately processed and identified with standard keys. Data were subjected to descriptive statistic and worm burdens recorded according to sex of worms, as well as breeds and sex of cattle. Analysis of variance (ANOVA) in general linear model on raw values was summarized as arithmetic means with standard errors of means. Mixed-model ANOVA verified relationship of worm burdens with cattle breed and sex. Nine Nematodes (Haemonchus contortus, Cooperia species, Trichostrongylus axei, Trichuris globulosa, Oesophagostomum radiatum, Nematodirus species, Ostertagia ostertagi, Dictyocaulus viviparus, Setaria labiatopapillosa ), two Trematodes (Calicophoron microbothrium and Fasciola gigantica), and one Cestode (Echinococcus granulosus Hydatid cysts) were recovered at necropsy. Total worm burden was 42252; commonest infections being Haemonchus contortus (40.9%) , Cooperia species (38.2%)  , and Trichostrongylus axei (9.5%) .  Female worms 28503(67.5%) established more than males 13644(32.3%). Hermaphrodites were only 105(0.3%) of total worm burden. Worm burden/cattle was 3018 (2966/bull and 3027/cow) but for cattle breeds, burdens were 3331/Namchi (600/Namchi bull and 4696/Namchi cow) and 2933/Gudali (5331/Gudali bull and 2693/Gudali cow).   Gudali breed showed more parasitic infections than Namchi, but the difference being statistically non-significant (P > 0.05). Worm burden/cattle was 3018 with an average of 2966/bull and 3027/cow but for cattle breeds, burdens were 3331/Namchi with and average of 600/Namchi bull and 4696/Namchi cow and 2933/Gudali with an average of 5331/Gudali bull and 2693/Gudali cow. Sex wise females recorded higher infection rates than males but the difference being statistically non-significant (P > 0.05). Hypobiotic larvae were not found in the digestas. Morphological identification of helminths has provided basis for further moléculaire identification to ensure sustainable helminthosis management intervention. Meanwhile strategic anti-helminth treatments will usefully control infections and re-infections in cattle in the area.

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Ebene, N. J., Njonka , D., Onyali, I. O., Manchang, T. K., & Ikpeze, O. O. (2022). SURVEY OF GASTROINTESTINAL AND PULMONARY PARASITISM IN TWO LOCAL CATTLE BREEDS IN THE HIGH GUINEA SAVANNAH, CAMEROON. The Bioscientist Journal, 10(2), 223-243. Retrieved from https://bioscientistjournal.com/index.php/The_Bioscientist/article/view/120
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References

Akkani, H., Jebali, J., Gharbi, M., Mahdhbi, M., Awadi, S. and Darghouth, M.A. (2013). Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia. Veterinary Parasitology, 193:118-125.
Baker . R.L(2011). Genetic resistance to endoparasites in sheep and goats. A review of genetic resistance to gastrointestinal nematode parasites in sheep and goats in the tropics and evidence for resistance in some sheep and goat breeds in sub-humid coastal Kenya. Animal Genetic Resources/Resources génétiques animales/Recursos genéticos animales. International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi, Kenya , Volume 24 , April 1998 , pp. 13 – 30.DOI: https://doi.org/10.1017/S1014233900001103
Belem, Adrien Marie Gaston., Ouédraogo, Ouinoaga Prosper and René Bessin. (2001). Gastro-intestinal nematodes and cestodes of cattle in Burkina Faso. Biotechnology and Agronomic Society of Environment, 5(1):17-21
Bishop S.C, Stear M.J. (2000). The use of a gamma-type function to assess the relationship between the number of adult Teladorsagia circumcincta and total egg output. Parasitology. 121:435–440. doi: 10.1017/S0031182099006526.
Boomker, J. (2014) Helminth infections of ruminants. Licensed under a Creative Commons Attribution license. (University of Pretoria; Afrivet; OIR Africa, an initiative of Saide; OIE, Collaborative Center for Training in: Integrative livestock and Wildlife Health and Management.
Chaudhry, U., Miller, M., Yazwinsky, T., Kaplan, R. and Gilleard, J. (2014). The presence of resistance mutations in Haemonchus placei from US cattle. Veterinary Parasitology, 29:411-415.
Chollet J.Y., Jacquiet Ph., Cardinate E., Ndamkou Ndamkou C., Diop C .,Thiam A .,Dorchies Ph . ( 2000) . Cooperia pectinata and Cooperia punctata, parasites of the abomasum of the cattle in northern Cameroon (Central Africa). Veterinary Parasitology, 88:.135-138.
Dineen, J. K. (1963). Immunological aspects of parasitism. Nature,197:268–269. (Doi:10.1038/197268a0)
Dunn. M. Angus. (1987). Veterinary Helminthology second Edition; William Henemann Medical Books LTD London WC1B 3HH
Ebangui, A. L., Erasmus, G. J., Mbah, D., Tawah, C. L. and Messine, O. (2001). Prediction of maternal performance from preweaning weight traits in Gudali beef in a tropical environment. Revista Portuguesa de Zootecnia AnoVII no (2001):33-42.
Ebene, N.J., Onyali, I.O., Mingoas, J.P., Pougue, H.B., Mfopit, M.Y., Katharina, Hergenroether., Manchang, T.K. and Nwosu, C.O. (2016). Efficacy Testing of Anthelmintics against Field strains of Trichostrongyles the Periurban Zone of Ngaoundere in Cameroon. Alexandria Journal of Veterinary Sciences, 50(1):78-86.
Galvani, A. P. (2003) Immunity, antigenic heterogeneity, and aggregation of helminth parasites. Journal.ofParasitology,89:232–241. (Doi:10.1645/0022-3395(2003)089[0232: IAHAAO]2.0.CO;2)
Ghasemikhah, R., Mirhendi, H., Kia, E.B., Mowlavi, Gh., Sarmadian, H., Meshgi, B., Golestan, B and Mobedi, I. (2011). Morphological and Morphometrical Description of Trichostrongylus Species Isolated from Domestic Ruminants in Khuzestan Province, Southwest Iran. Iran Journal of Parasitology, 6(3): 82–88. PMCID: PMC3279896
Gasbarre, L.C., and Miller, J.E. (2000). Genetics of helminth resistance. In Breeding for disease resistance in farm animals (ed. R.F.E. Axford, S.C. Bishop, F.W. Nicholas, and J.B. Owen), pp. 129-152. CABI Publishing, Wallingford.
Gasbarre, L.C., Smith, L.L., Hoberg, E.P. and Pillitt, P.A. (2009a). Further characterization of a cattle nematode population with demonstrated resistance current to current anthelmintics. Veterinary Parasitology,166:275-280.
Gasbarre, L.C., Smith, L.L., Lichtenfels, J.R. and Pillitt, P.A. (2009b). The identification of nematode parasites resistant to multiple classes of anthelmintics in a commercial cattle population in the US. Veterinary Parasitology,166:281-285.
Grillo, V., Jackson, F., Cabaret, J. and Gilleard, J.S. (2007). Population genetic analysis of the ovine parasitic nematode Teladorsagia circomcincta and evidence for a cryptic species. International Journal for Parasitology, 37:435-447.
Hendix, C. (1998). Diagnosis of veterinary parasitology, 2nd edition. Mosby, London, pp 49
Hoberg, E.P., Lichtenfels, J.R. and Gibbons, L. (2004). Phylogeny for species of Haemonchus (Nematoda: Trichostrongylidae) considerations of their evolutionary history and global biogeography among Camelidae and Pecora (Artyodactyla). Journal of Parasitology, 90:1085-1102.
Hogg, R., Whitaker, K., Collins, R., Holmes, P., Mitchel, S., Anscombe, J., Redman, L. and Gilleard, J. (2010). Haemonchosis in large ruminants in the UK. Veterinary Record, 166: 373-374.
Ikpeze, O.O. (2009). Arrested development in nematodes of grazing ruminants: a review. International Journal of Zoology, 1(1):25-31.
ILRI (International Livestock Research Institute). (1997). Out of Africa, into a global mandate. ILRI, Nairobi, Kenya, pp 54.
IRZ/GTZ. (1989). Livestock farming systems in Adamaoua, Research Report no I. Wakwa, Cameroon.96p+ annexes
Kassai, T. (1999). Veterinary Helminthology. Butterworth-Heinemann. Reed Educational and Professional Publishing Ltd., Oxford, USA.260pp.
Kaufman, J and Pfister, K. (1990). The seasonal epidemiology of gastrointestinal nematode in N’dama cattle in the Gambia. Veterinary Parasitology. 37 : 45-54.
Krishnan, L., Guilbert , L. J., Russell A. S., Wegmann, T. G., Mosmann , T. R., Belosevic, M. (1996) Pregnancy impairs resistance of C57BL/6 mice to Leishmania major infection and causes decreased antigen-specific IFN-gamma responses and increased production of T helper 2 cytokines. Journal of Immunology, 156(2) :644–652
Levine, N. (1980). Nematode parasites of domestic animals and man, 2nd ed. Minneapolis, MN: Burgess Publishing Co, pp 477.
Lichtenfels, J.R., Pilitt, P.A., Le Jambre, L.F. (1988a). Spicule lengths of the stomach nematodes of ruminants, Haemonchus contortus, Haemonchus placei and their hybrids. of Proceedings. Helminthology. Society. Wash, 55:97–100.
Lichtenfels, J.R., Pillitt, P.A, and Hoberg, E.P.(1994). New morphological characters for identifying individual specimens of Haemonchus spp. (Nematoda: Trichostrongyloidae) and a key to species of ruminants of North America. Journal of Parasitology, 80:107-119.
Lloyd, S. (1983). Effect of pregnancy and lactation upon infection. Veterinary Immunology Immunopathology 4(1–2):153–176. https://doi.org/10.1016/0165-2427(83)90057-0
Maharana, B. R., Binod, Kumar, N. R. , Sudhakar, S. K. , Behera, and T. K. Patbandha. (2016). Prevalence of gastrointestinal parasites in bovines in and around Junagadh (Gujarat). Journal Parasitology Dis. Dec; 40(4): 1174–1178. Published online 2015 Feb 14. doi: 10.1007/s12639-015-0644-6; PMCID: PMC5118270; PMID: 27876909
Martin, L, B., Weil, Z. M., Nelson, R. J. (2008). Seasonal changes in vertebrate immune activity: mediation by physiological trade-offs. Philos Trans R Society B-Biology Sciences 363(1490):321–339. https://doi.org/10.1098/rstb.2007.2142
MINEPIA (2003). Annual Report
Mungube, E O., Wamae, LW., Omondi, G. A., and Mwangi, G. (2015). Prevalence of multiple resistant Haemonchus and Ostertagia species in goats and cattle in Machakos, Eastern Kenya.
Ndamukong, K.J.N, and Sewell, M.M.H. (1992). Resistance to benzimidazote anthelmintics by Trichostrongyles in sheep and goats in North-West Cameroun. Veterinary Parasitology, 41:335-339
Ngole, I.U., Ndamukong, K.J, and Mbuh, J.V. (2003). Internal parasites and haematological values in cattle slaughtered in Buea Subdivision of Cameroon. Tropical Animal Health and Production, 35:409-413.
Ntonifor, H. N., Shei, S. J., Ndaleh, N. W., and Mbunkur, G. N.( 2013). Epidemiological studies of gastrointestinal parasitic infections in ruminants in Jakiri, Bui Division, Northwest Region of Cameroon. Journal of Veterinary Medicine and Animal Health ,5(12): 344-352; DOI: 10.5897/JVMAH2013.0209;© 2013 Academic Journals.
Pandey, V.S., Chitate, F, and Nyanzunda, T.M., (1993). Epidemiological observations on gastro-intestinal nematodes in communal land cattle from the highveld of Zimbabwe,’ Veterinary Parasitology 51, 99–106. http://dx.doi.org/10.1016/0304- 4017(93)90200-7
Pfukenyi, D.M., Mukaratirwa, S., Willingham, A.L. & Monrad, J. (2007). ‘Epidemiological studies of parasitic gastrointestinal nematodes, cestodes and coccidia infections in cattle in the highveld and lowveld communal grazing areas of Zimbabwe,’ Onderstepoort Journal of Veterinary Research ,74 : 129–142. http://dx.doi. Org/10.4102/ojvr. v74i2.132, PMid:17883199
Poulin, R. (1996). Helminth growth in vertebrate hosts : does host sex matter ? International Journal Parasitology, 26(11) :1311–1315. https://doi.org/10.1016/s0020-7519(96)00108-7
Rommel, M., Eckert, J., Kutzer, E., Korting, W. and Schneider, T. (2000). Veterinar Medical Parasitology. 5th edition. Parey Buchverlag Berlin, Germany.
Seaton, D. S., Jackson, F., Smith, W. D. & Angus, K. W. (1989). Development of immunity to incoming radiolabelled larvae in lambs continuously infected with Ostertagia circumcincta. Resource. Veterinary. Sciences. 46: 241–246.
Schell, S.C. (1970). How to know the Trematodes. W.C. Brown Co., Dubuque, Iowa.
Stear, M. J., Bairden, K., Duncan, J. L., Gettinby, G., McKellar, Q. A., Murray, M. and Wallace, D. S. (1995) The distribution of faecal nematode egg counts in Scottish Blackface lambs following natural, predominantly Ostertagia circumcincta infection. Parasitology 110, 573–581.
Stear, M. J., Bairden, K., Bishop, S.C., Gettinby, G., McKellar, Q. A., Park, M., Strain, S. A. J. and Wallace, D. S.(1998). The processes influencing the distribution of parasitic nematodes among naturally infected lambs. Parasitology, 117:165–171. (Doi :10.1017/S0031182098002868)
Stear, Michael J., Lesley, Fitton, Giles, T. Innocent, Lisa Murphy., Kerry , Rennie, and Louise Matthews. ( 2007). The dynamic influence of genetic variation on the susceptibility of sheep to gastrointestinal nematode infection. Journal of. Resource. Society. Interface (2007) 4, 767–776 doi:10.1098/rsif.2007.1104
T0756e04.htm. FAO files. Chapter 3 (Continued). Parasitic diseases. Diseases caused by helminths
Taylor, M.A., Coop, R.L. and Wall, R.L. (2007). Veterinary Parasitology, Third Edition. Blackwell Publishing Professional, 2121 State Avenue, Ames, Iowa 50014-8300, USA, pp 874
Taylor, M.A., Coop, R.L. and Wall, R.L. (2016). Veterinary Parasitology, Fourth Edition. Blackwell Publishing Professional, 2121 State Avenue, Ames, Iowa 50014-8300, USA. pp 1006

Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and. Jennings, F.W. (1996). Veterinary Parasitology, 2nd edition, pp 196. Blackwell Science Ltd, Blackwell Publishing Company, Oxford, UK. pp. 1-138.
Vassilev, G.D. (1999). ‘Prevalence of internal parasite infections of cattle in the communal farming areas of Mashonaland East Province, Zimbabwe.’ Zimbabwe Veterinary Journal, 30:1–17.
Waller, P.J. (1997). Sustainable helminth control of ruminants in developing countries. Veterinary Parasitology, 7:195-207.
Wymann Monica N., Bassirou Bonfoh., Koniba Traore., Saïdou Tembely and Jacok Zinsstag. (2006). Species diversity acquisition of gastrointestinal parasites in calves ages 0-13 months in peri-urban livestock production in Mali. Elsevier B.V. All rights reserved.
Wyrobisz, A., Kowal, J. and Nosal. (2015). Insight into species diversity of the trichostrongylidae Leiper, 1912 (Nematoda: Strongylida) in ruminants. Journal of Helminthology, 1-8.doi:10.1017/S0022149X15001017
Zajac M. Anne and Conboy A. Gary. (2012). Veterinary Clinical Parasitology Eighth Edition,Under the auspices of the American Association of Veterinary Parasitologists. John Wiley & Sons Ltd. The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK
Zarlenga, D.S., Hoberg, E.P., Tuo, W. (2016). The Identification of Haemonchus Species and Diagnosis of Haemonchosis. In: Gasser, R.B., von Samson-Himmelstjerna, G., (Eds.), Haemonchus contortus and Haemonchosis – Past, Present and Future Trends, 145–180.ISBN: 9780128103951
Zinsstag J. (2000). Gastrointestinal nematodes of N’dama cattle in The Gambia: Effects on productivity and options for control. Ph. D. thesis No.11. Prince Leopold Institute of Tropical Medicine Antwerpen, Belgium.
Zinsstag, J., Ankers, J., Demfley, L., Njie, M., Kaufman, J., Itty, P., Pfister, K. and Pandey, S. (1997). Effect of strategic gastrointestinal control on growth of Ndama cattle in Gambia. Veterinary parasitology, 68:143-153.
Zinsstag, J., Ankers, P., Ndao, M., Bonfoh, B. and Pfister, V.S. (1998). Multiparasitism, production and economics in domestic animals in Subsaharan West Africa. Parasitology Today, 14:46-49.